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l3000015 n2 r d systems  (R&D Systems)


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    R&D Systems l3000015 n2 r d systems
    L3000015 N2 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/l3000015+n2+r+d+systems/N-2+MAX+Media+Supplement+(100X)/pm37405912-231-230-232
    Average 95 stars, based on 101 article reviews
    l3000015 n2 r d systems - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PLZF Santa Cruz Cat# sc-28319; RRID:AB_2218941 KIT R&D Systems Cat# AF1356; RRID:AB_354750 SLC7A5 Cell Signaling Technology Cat# 5347S; RRID:AB_10695104 SLC38A2 Sigma Aldrich Cat# HPA035180; RRID:AB_10669901 SLC2A1 Abcam Cat# ab115730; RRID:AB_10903230 gH2AX Millpore Cat# 05-636; RRID:AB_309864 VASA R&D Systems Cat# AF2030; RRID:AB_2277369 P70S6K Cell Signaling Technology Cat# 9234P; RRID:AB_2269803 p-70S6K Cell Signaling Technology Cat# 9202S; RRID:AB_331676 S6 Cell Signaling Technology Cat# 4858P; RRID:AB_916156 p-S6 Cell Signaling Technology Cat# 2217S; RRID:AB_331355 4EBP1 Cell Signaling Technology Cat# 2855P; RRID:AB_560835 p-4EBP1 Cell Signaling Technology Cat# 9644T; RRID:AB_2097841 FLAG Sigma Aldrich Cat# F1804; RRID:AB_262044 a6 integrin BD Biosciences Cat# 555736; RRID:AB_396079 KIT BD Biosciences Cat# 553356; RRID:AB_398536 donkey anti-mouse IgG Alexa Flour 488 Life Technologies Cat# A21202; RRID:AB_141607 donkey anti-rabbit IgG Alexa Flour 568 Life Technologies Cat# A10042; RRID:AB_2534017 donkey anti-goat IgG Alexa Flour 647 Life Technologies Cat# A21447; RRID:AB_141844 Biological samples Human testis testicular tissue from organ donors Center for Organ Recovery and Education N/A Chemicals, Peptides, and Recombinant Proteins Superscript II Reverse Transcriptase Thermo Fisher Scientific Cat# 18080051 NuPage 4 %–12 %Bis-Tris Protein Gel Thermo Fisher Scientific Cat# NP0322BOX cOmplete, EDTA-Free Roche Cat# 4 693 132 RIPA Buffer Thermo Cat# 89900 all-transRA Sigma-Aldrich Cat# R2625 2-NBDG Sigma-Aldrich Cat# 72987 Glucose-Cy5 Sigma-Aldrich Cat# SML3233 collagenase IV Thermo Fisher Scientific Cat# 17104019 TRIzol Invitrogen Cat# 15596018 Power SYBR Green Master Mix Applied Biosystems Cat# A25742 Lipofectamine 3000 Invitrogen Cat# L3000015 N2 R&D Systems Cat# AR-009 Pyruvic Acid Sigma-Aldrich Cat# P2256 D-(+)-glucose Sigma-Aldrich Cat# G7021 DL-Lactic Acid Sigma-Aldrich Cat# L4263 BSA MPBiomedicals Cat# 810661 L-Glutamine Sigma-Aldrich Cat# G7513 2-Mercaptoethanol Sigma-Aldrich Cat# M3148 MEM Vitamin Invitrogen Cat# 11120-052 NEAA Invitrogen Cat# 11140-050 (Continued on next page) 16 Cell Reports 42, 112749, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ascorbic Acid Sigma-Aldrich Cat# A4544 d-Biotin Sigma-Aldrich Cat# B4501 Beta-Estradiol Sigma-Aldrich Cat# E2758 FBS Hyclone Cat# SH30396.03 KSR Invitrogen Cat# 10828-028 Human FGF2 Peprotech Cat# 100-18B Rat GDNF Peprotech Cat# 450-51 Critical commercial assays Slc7a5 probe RNAscope Probe Cat# 461031 Slc38a2 probe RNAscope Probe Cat# 1184001-C2 Slc2a1 probe RNAscope Probe Cat# 458671-C2 TruSeq RNA Sample Preparation Kit v2 Illumina Cat# RS-122-2001 Illumina ChIP-Seq Sample Prep Kit Illumina Cat# FC-102-1003 Illumina Sequencing Kit v4 Illumina Cat# 15003926 Illumina TruSeq ChIP Sample Preparation Kit Illumina Cat# IP-202-1012 Chromium Single Cell 30 v3 Reagent Kits & Chip Kits 10x Genomics Cat# PN-1000075 Seahorse XF Mito Fuel Flex Agilent Cat# 103260-100 Glutamine/glutamate-Glo Assay kit Promega J8021 NovaRed Kit Vector Laboratories SK-4800 DC protein assay BioRad Cat# 5000111 Deposited Data RNA-Seq of WT, Meiosin KO and Stra8 KO Testes Ishiguro et al.12 DDBJ DRA007066 ChIP-Seq of MEIOSIN Ishiguro et al.12 DDBJ DRA007778 RNA-Seq and ChIP-Seq of GS Cells Ishiguro et al.12 GEO GSE116798 STRA8-FLAG ChIP-Seq Data Kojima et al.11 GEO GSE115928 Raw and analyzed 10x Genomics scRNA-seq datasets This paper GEO GSE211390 In vitro meiosis 10x Genomics scRNA-seq Zhang et al.15 GEO GSE153300 Mouse testis Slide-seq Chen et al.26 PRJNA668433 Experimental models: Cell lines Cultured mouse spermatogonial stem cells This paper N/A Experimental models: organisms/strains Mouse: C57BL/6J Charles River Laboratory Strain code: 027 Mouse: Stra8Knockout The Jackson Laboratory JAX: 023805 MEF R&D systems Cat# PSC001 Oligonucleotides Slc7a5_siRNA Dharmacon Cat# 041166-01-0005 Slc38a2_siRNA Dharmacon Cat# 059380-00-0005 Slc2a1_siRNA Dharmacon Cat# 044254-02-0005 Ctrl_siRNA Dharmacon Cat# D-001210-01-05 Primers for qRT-PCR This paper Table S1 Recombinant DNA Stra8-flag This paper N/A Stra8D1 This paper N/A Stra8D2 This paper N/A Stra8D3 This paper N/A Stra8DC This paper N/A (Continued on next page) Cell Reports 42, 112749, July 25, 2023 17

    Reverse Transcription:

    Article Title: Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PLZF Santa Cruz Cat# sc-28319; RRID:AB_2218941 KIT R&D Systems Cat# AF1356; RRID:AB_354750 SLC7A5 Cell Signaling Technology Cat# 5347S; RRID:AB_10695104 SLC38A2 Sigma Aldrich Cat# HPA035180; RRID:AB_10669901 SLC2A1 Abcam Cat# ab115730; RRID:AB_10903230 gH2AX Millpore Cat# 05-636; RRID:AB_309864 VASA R&D Systems Cat# AF2030; RRID:AB_2277369 P70S6K Cell Signaling Technology Cat# 9234P; RRID:AB_2269803 p-70S6K Cell Signaling Technology Cat# 9202S; RRID:AB_331676 S6 Cell Signaling Technology Cat# 4858P; RRID:AB_916156 p-S6 Cell Signaling Technology Cat# 2217S; RRID:AB_331355 4EBP1 Cell Signaling Technology Cat# 2855P; RRID:AB_560835 p-4EBP1 Cell Signaling Technology Cat# 9644T; RRID:AB_2097841 FLAG Sigma Aldrich Cat# F1804; RRID:AB_262044 a6 integrin BD Biosciences Cat# 555736; RRID:AB_396079 KIT BD Biosciences Cat# 553356; RRID:AB_398536 donkey anti-mouse IgG Alexa Flour 488 Life Technologies Cat# A21202; RRID:AB_141607 donkey anti-rabbit IgG Alexa Flour 568 Life Technologies Cat# A10042; RRID:AB_2534017 donkey anti-goat IgG Alexa Flour 647 Life Technologies Cat# A21447; RRID:AB_141844 Biological samples Human testis testicular tissue from organ donors Center for Organ Recovery and Education N/A Chemicals, Peptides, and Recombinant Proteins Superscript II Reverse Transcriptase Thermo Fisher Scientific Cat# 18080051 NuPage 4 %–12 %Bis-Tris Protein Gel Thermo Fisher Scientific Cat# NP0322BOX cOmplete, EDTA-Free Roche Cat# 4 693 132 RIPA Buffer Thermo Cat# 89900 all-transRA Sigma-Aldrich Cat# R2625 2-NBDG Sigma-Aldrich Cat# 72987 Glucose-Cy5 Sigma-Aldrich Cat# SML3233 collagenase IV Thermo Fisher Scientific Cat# 17104019 TRIzol Invitrogen Cat# 15596018 Power SYBR Green Master Mix Applied Biosystems Cat# A25742 Lipofectamine 3000 Invitrogen Cat# L3000015 N2 R&D Systems Cat# AR-009 Pyruvic Acid Sigma-Aldrich Cat# P2256 D-(+)-glucose Sigma-Aldrich Cat# G7021 DL-Lactic Acid Sigma-Aldrich Cat# L4263 BSA MPBiomedicals Cat# 810661 L-Glutamine Sigma-Aldrich Cat# G7513 2-Mercaptoethanol Sigma-Aldrich Cat# M3148 MEM Vitamin Invitrogen Cat# 11120-052 NEAA Invitrogen Cat# 11140-050 (Continued on next page) 16 Cell Reports 42, 112749, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ascorbic Acid Sigma-Aldrich Cat# A4544 d-Biotin Sigma-Aldrich Cat# B4501 Beta-Estradiol Sigma-Aldrich Cat# E2758 FBS Hyclone Cat# SH30396.03 KSR Invitrogen Cat# 10828-028 Human FGF2 Peprotech Cat# 100-18B Rat GDNF Peprotech Cat# 450-51 Critical commercial assays Slc7a5 probe RNAscope Probe Cat# 461031 Slc38a2 probe RNAscope Probe Cat# 1184001-C2 Slc2a1 probe RNAscope Probe Cat# 458671-C2 TruSeq RNA Sample Preparation Kit v2 Illumina Cat# RS-122-2001 Illumina ChIP-Seq Sample Prep Kit Illumina Cat# FC-102-1003 Illumina Sequencing Kit v4 Illumina Cat# 15003926 Illumina TruSeq ChIP Sample Preparation Kit Illumina Cat# IP-202-1012 Chromium Single Cell 30 v3 Reagent Kits & Chip Kits 10x Genomics Cat# PN-1000075 Seahorse XF Mito Fuel Flex Agilent Cat# 103260-100 Glutamine/glutamate-Glo Assay kit Promega J8021 NovaRed Kit Vector Laboratories SK-4800 DC protein assay BioRad Cat# 5000111 Deposited Data RNA-Seq of WT, Meiosin KO and Stra8 KO Testes Ishiguro et al.12 DDBJ DRA007066 ChIP-Seq of MEIOSIN Ishiguro et al.12 DDBJ DRA007778 RNA-Seq and ChIP-Seq of GS Cells Ishiguro et al.12 GEO GSE116798 STRA8-FLAG ChIP-Seq Data Kojima et al.11 GEO GSE115928 Raw and analyzed 10x Genomics scRNA-seq datasets This paper GEO GSE211390 In vitro meiosis 10x Genomics scRNA-seq Zhang et al.15 GEO GSE153300 Mouse testis Slide-seq Chen et al.26 PRJNA668433 Experimental models: Cell lines Cultured mouse spermatogonial stem cells This paper N/A Experimental models: organisms/strains Mouse: C57BL/6J Charles River Laboratory Strain code: 027 Mouse: Stra8Knockout The Jackson Laboratory JAX: 023805 MEF R&D systems Cat# PSC001 Oligonucleotides Slc7a5_siRNA Dharmacon Cat# 041166-01-0005 Slc38a2_siRNA Dharmacon Cat# 059380-00-0005 Slc2a1_siRNA Dharmacon Cat# 044254-02-0005 Ctrl_siRNA Dharmacon Cat# D-001210-01-05 Primers for qRT-PCR This paper Table S1 Recombinant DNA Stra8-flag This paper N/A Stra8D1 This paper N/A Stra8D2 This paper N/A Stra8D3 This paper N/A Stra8DC This paper N/A (Continued on next page) Cell Reports 42, 112749, July 25, 2023 17

    SYBR Green Assay:

    Article Title: Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PLZF Santa Cruz Cat# sc-28319; RRID:AB_2218941 KIT R&D Systems Cat# AF1356; RRID:AB_354750 SLC7A5 Cell Signaling Technology Cat# 5347S; RRID:AB_10695104 SLC38A2 Sigma Aldrich Cat# HPA035180; RRID:AB_10669901 SLC2A1 Abcam Cat# ab115730; RRID:AB_10903230 gH2AX Millpore Cat# 05-636; RRID:AB_309864 VASA R&D Systems Cat# AF2030; RRID:AB_2277369 P70S6K Cell Signaling Technology Cat# 9234P; RRID:AB_2269803 p-70S6K Cell Signaling Technology Cat# 9202S; RRID:AB_331676 S6 Cell Signaling Technology Cat# 4858P; RRID:AB_916156 p-S6 Cell Signaling Technology Cat# 2217S; RRID:AB_331355 4EBP1 Cell Signaling Technology Cat# 2855P; RRID:AB_560835 p-4EBP1 Cell Signaling Technology Cat# 9644T; RRID:AB_2097841 FLAG Sigma Aldrich Cat# F1804; RRID:AB_262044 a6 integrin BD Biosciences Cat# 555736; RRID:AB_396079 KIT BD Biosciences Cat# 553356; RRID:AB_398536 donkey anti-mouse IgG Alexa Flour 488 Life Technologies Cat# A21202; RRID:AB_141607 donkey anti-rabbit IgG Alexa Flour 568 Life Technologies Cat# A10042; RRID:AB_2534017 donkey anti-goat IgG Alexa Flour 647 Life Technologies Cat# A21447; RRID:AB_141844 Biological samples Human testis testicular tissue from organ donors Center for Organ Recovery and Education N/A Chemicals, Peptides, and Recombinant Proteins Superscript II Reverse Transcriptase Thermo Fisher Scientific Cat# 18080051 NuPage 4 %–12 %Bis-Tris Protein Gel Thermo Fisher Scientific Cat# NP0322BOX cOmplete, EDTA-Free Roche Cat# 4 693 132 RIPA Buffer Thermo Cat# 89900 all-transRA Sigma-Aldrich Cat# R2625 2-NBDG Sigma-Aldrich Cat# 72987 Glucose-Cy5 Sigma-Aldrich Cat# SML3233 collagenase IV Thermo Fisher Scientific Cat# 17104019 TRIzol Invitrogen Cat# 15596018 Power SYBR Green Master Mix Applied Biosystems Cat# A25742 Lipofectamine 3000 Invitrogen Cat# L3000015 N2 R&D Systems Cat# AR-009 Pyruvic Acid Sigma-Aldrich Cat# P2256 D-(+)-glucose Sigma-Aldrich Cat# G7021 DL-Lactic Acid Sigma-Aldrich Cat# L4263 BSA MPBiomedicals Cat# 810661 L-Glutamine Sigma-Aldrich Cat# G7513 2-Mercaptoethanol Sigma-Aldrich Cat# M3148 MEM Vitamin Invitrogen Cat# 11120-052 NEAA Invitrogen Cat# 11140-050 (Continued on next page) 16 Cell Reports 42, 112749, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ascorbic Acid Sigma-Aldrich Cat# A4544 d-Biotin Sigma-Aldrich Cat# B4501 Beta-Estradiol Sigma-Aldrich Cat# E2758 FBS Hyclone Cat# SH30396.03 KSR Invitrogen Cat# 10828-028 Human FGF2 Peprotech Cat# 100-18B Rat GDNF Peprotech Cat# 450-51 Critical commercial assays Slc7a5 probe RNAscope Probe Cat# 461031 Slc38a2 probe RNAscope Probe Cat# 1184001-C2 Slc2a1 probe RNAscope Probe Cat# 458671-C2 TruSeq RNA Sample Preparation Kit v2 Illumina Cat# RS-122-2001 Illumina ChIP-Seq Sample Prep Kit Illumina Cat# FC-102-1003 Illumina Sequencing Kit v4 Illumina Cat# 15003926 Illumina TruSeq ChIP Sample Preparation Kit Illumina Cat# IP-202-1012 Chromium Single Cell 30 v3 Reagent Kits & Chip Kits 10x Genomics Cat# PN-1000075 Seahorse XF Mito Fuel Flex Agilent Cat# 103260-100 Glutamine/glutamate-Glo Assay kit Promega J8021 NovaRed Kit Vector Laboratories SK-4800 DC protein assay BioRad Cat# 5000111 Deposited Data RNA-Seq of WT, Meiosin KO and Stra8 KO Testes Ishiguro et al.12 DDBJ DRA007066 ChIP-Seq of MEIOSIN Ishiguro et al.12 DDBJ DRA007778 RNA-Seq and ChIP-Seq of GS Cells Ishiguro et al.12 GEO GSE116798 STRA8-FLAG ChIP-Seq Data Kojima et al.11 GEO GSE115928 Raw and analyzed 10x Genomics scRNA-seq datasets This paper GEO GSE211390 In vitro meiosis 10x Genomics scRNA-seq Zhang et al.15 GEO GSE153300 Mouse testis Slide-seq Chen et al.26 PRJNA668433 Experimental models: Cell lines Cultured mouse spermatogonial stem cells This paper N/A Experimental models: organisms/strains Mouse: C57BL/6J Charles River Laboratory Strain code: 027 Mouse: Stra8Knockout The Jackson Laboratory JAX: 023805 MEF R&D systems Cat# PSC001 Oligonucleotides Slc7a5_siRNA Dharmacon Cat# 041166-01-0005 Slc38a2_siRNA Dharmacon Cat# 059380-00-0005 Slc2a1_siRNA Dharmacon Cat# 044254-02-0005 Ctrl_siRNA Dharmacon Cat# D-001210-01-05 Primers for qRT-PCR This paper Table S1 Recombinant DNA Stra8-flag This paper N/A Stra8D1 This paper N/A Stra8D2 This paper N/A Stra8D3 This paper N/A Stra8DC This paper N/A (Continued on next page) Cell Reports 42, 112749, July 25, 2023 17



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    l3000015 n2 r d systems  (R&D Systems)
    95
    R&D Systems l3000015 n2 r d systems
    L3000015 N2 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/l3000015+n2+r+d+systems/N-2+MAX+Media+Supplement+(100X)/pm37405912-231-230-232
    Average 95 stars, based on 1 article reviews
    l3000015 n2 r d systems - by Bioz Stars, 2026-11
    95/100 stars
    		  Buy from Supplier

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